A Three-Stemmed mRNA Pseudoknot in the SARS Coronavirus Frameshift Signal
نویسندگان
چکیده
منابع مشابه
A Three-Stemmed mRNA Pseudoknot in the SARS Coronavirus Frameshift Signal
A wide range of RNA viruses use programmed -1 ribosomal frameshifting for the production of viral fusion proteins. Inspection of the overlap regions between ORF1a and ORF1b of the SARS-CoV genome revealed that, similar to all coronaviruses, a programmed -1 ribosomal frameshift could be used by the virus to produce a fusion protein. Computational analyses of the frameshift signal predicted the p...
متن کاملDevelopment of a quantitative assay for SARS coronavirus and correlation of GAPDH mRNA with SARS coronavirus in clinical specimens.
AIMS To develop a quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR) for severe acute respiratory syndrome coronavirus (SARS-CoV) detection and explore the potential of using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control to exclude false negative results. METHODS SARS-CoV and GAPDH mRNA were both measured in 26 specimens from 16 patients wit...
متن کاملIn Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation
SARS-coronavirus (SARS-CoV) genome expression depends on the synthesis of a set of mRNAs, which presumably are capped at their 5' end and direct the synthesis of all viral proteins in the infected cell. Sixteen viral non-structural proteins (nsp1 to nsp16) constitute an unusually large replicase complex, which includes two methyltransferases putatively involved in viral mRNA cap formation. The ...
متن کاملAn atypical RNA pseudoknot stimulator and an upstream attenuation signal for −1 ribosomal frameshifting of SARS coronavirus
The -1 ribosomal frameshifting requires the existence of an in cis RNA slippery sequence and is promoted by a downstream stimulator RNA. An atypical RNA pseudoknot with an extra stem formed by complementary sequences within loop 2 of an H-type pseudoknot is characterized in the severe acute respiratory syndrome coronavirus (SARS CoV) genome. This pseudoknot can serve as an efficient stimulator ...
متن کاملNew Frameshifting Pseudoknot Found in SARS Virus
0929 IM model has required the assumption that all of the populations were constant in size, and therefore it has not been useful for assessing how descendant populations arose or changed in size. Hey analyzed DNA sequences from nine loci, so that the population genetic history could be found despite the variation among genes. He also added an additional parameter to the standard IM model to in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS Biology
سال: 2005
ISSN: 1545-7885
DOI: 10.1371/journal.pbio.0030172